Epidemiological Study of Some Candida albicans Strains in Patients Undergoing Chemotherapy by Using PCR Technique in Whole Blood

Abstract

Candida species were common human commensals that can cause a wide spectrum of disease, major concern was hematogenously disseminated infection which was occurring with increased prevalence in immunocompromised patients, although Candida albicans remains the most frequent cause of fungemia and hematogenously disseminated candidiasis. In the presented study was 102 patient undergoing chemotherapy and 73 control outpatients resuming Tikrit teaching hospital were examined to determine the prevalence of candidemia by using blood culture method from isolation, cultural identification, were 17.6% from cancer patients undergoing chemotherapy positive for candidemia, while all control patient negative. Were83.4% from rural area for province of Tikrit . in addition, were 61.2% from south direction Tikrit city and DNA extraction from whole blood samples depending on distribution cancer patients and candidemia to detected direct diagnosis and universal strains in our epidemiological study, were 80% Candida albicans strains of ATCC 10261, 3153, A72, MEN, Cl032, ClOll, Cl012, Cl035, ClO60, and ClO61 by using PCR technique.