Detection of CD3,19 and CD4,8 markers on lymphocyte of lymphocytic leukemia Iraqi patients

Abstract

This work aimed to study expression of CD marker on surface of lymphocyte of (acute lymphocyte leukemia ALL; chronic lymphocyte leukemia CLL, and control). To achieve this goal, blood samples were collected from 39 leukemic cases (21 ALL and 18 CLL), and isolation of lymphocyte than proliferation of leukemia lymphocyte culture for detection of CD Marker on surface of lymphocyte by immunofluorescence technique, the results, appeared elevated levels of CD8+ cells in both ALL and CLL patients were detected, in contrast, lower levels of CD4+ cells and CD4/CD8 ratio were noticed in these patients. However, high level of CD3+ cells, CD8/CD19 ratio and CD19+ cells were noticed in ALL patients and low level in CLL patients.