Prevalence of Cytomegalovirus Antibodies among blood donors in Mosul Central Blood Bank/Iraq

Abstract

Cytomegalovirus infection is a matter of concern for blood bank professionals and blood transfusion recipients, especially incase of transfusion to neonates and immunocompromised patients, seroprevalence study was conduct on 100 blood samples from donors that were used in serological screening and confirm by real time PCR. All analysis were conducted using the Elisa technique (Biotechch, Inc. USA) and Real Time Polymerase Chain Reaction technique (Sacace, Italy). Interpretation results accordance with the manufacturer's instructions, from beginning May to the end October 2011. a total of 100 blood samples were collected from voluntary blood donors, the samples against anti-CMV-IgM and IgG using Elisa technique and determination CMV DNA viral load by Real Time PCR, thereals shows seroprevalence of anti-CMV IgG in samples studied was 64% and that of anti-CMV IgM 3%, while the CMV DNA was 2%. The viral load more than 80000 copy/ml.