Genetic polymorphism of FTO and MC4R genes in Type 2 Diabetes Mellitus patients with and without obesity

Abstract

Diabetes can be considered one of the greatest prevalent health problems in the world, and obesity is one of the main causes of diabetes. The main objective of this investigation was to explore the frequency of both alpha-ketoglutarate dependent dioxygenase (FTO) gene_ rs9939609 and melanocortin 4 receptor (MC4R) gene - rs17782313 in diabetes patient with and without obesity.: We enrolled 39 individuals as a control group and 102 as diabetes group (T2DM without obesity (N=52) and T2DM with obesity (N=50)). Genetic analysis was carry out by Tetra Primer of amplification refractory mutation system (ARMS-PCR) for both FTO SNP rs9939609 and MC4R gene rs17782313. FTO rs9939609 polymorphism between control group and diabetes patients was show significant associations. FTO rs9939609 A-A /A-T have highly significantly associated between healthy group with both T2DM without obesity , and T2DM with obesity in all inheritances model except in over dominance model. MC4R ( rs17782313) gene C-C/C-T have significantly related between healthy group with both T2DM without obesity, and T2DM with obesity in Codominant and Recessive model, while no significantly associated in Dominant and Over dominant model. Our research suggested that rs9939609 in the FTO gene and rs12970134 in the MC4R gene are consider risk candidate factors in patients with diabetes. In diabetic individuals without obesity, the correlation was highly significant.