Co-precipitation method of superparamagnetic nanoparticles against some bacteria applications

Abstract

Due to the exponential diversity of effective applications of superparamagnetic iron oxide nanoparticles (SPIONs) in various biological applications, the synthesis process of magnetic nanoparticles is crucial for the fabrication of small and stable particles and their successful application. In this work, we report the synthesis of a co-precipitation method based on sodium hydroxide (NaOH)-mediated precipitation of $\text{Fe}^{3+}$ and $\text{Fe}^{2+}$ salts in aqueous solution using trisodium citrate as surfactant in a closed system It was carried out under an inert atmosphere of nitrogen. atmosphere. He resulting synthesized SPIONs were characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray powder diffraction (XRD), and vibrating sample magnetometer (VSM) and zeta potential (Zp) analysis. TEM and SEM images showed that the particles were spherical and the particle size was $\ge 20\text{ nm}$. XRD pattern during analysis showing the presence of peaks corresponding to the magnetite $\text{Fe}_3\text{O}_4$ phase of a sample technique Magnetic Vibration VSM It was found that the unwrapped magnetic nanoparticles coated with SPIONs–PEI polymer possess magnetic properties but it decreases with increasing PEI concentration by 20% which is sufficient to respond to the magnetic. For the application of nanoparticles uncoated SPIONs and encapsulated SPIONs–PEI were applied to the biological activities to inhibit the growth of bacteria (E. coli, P. mirabilis, P. aeruginosa, S. aureus), which showed a significant effect on inhibiting the growth of bacteria with gram-positive and gram-negative bacteria (P.