DETECTION OF THE EFFECT OF HuLK ON THE 28S GENE OF BEMISIA TABACI USING DNA SEQUENCING

Abstract

This study aims to identify the effect of some types of insecticides on the genetic characteristics of the whitefly, while determining the resulting mortality rate. The experimental environment was then secured by covering the filters with a layer of air- and water-permeable tulle, and then spraying the whitefly and plants with the American insecticide "Hulk" produced by Ancestex at the doses indicated on the cover. Three treatments were carried out with a time interval of 20 days between each treatment. After each treatment, fly samples were collected to extract DNA from them, and to study genetic changes by comparing them with the control sample using polymerase chain reaction (PCR) and genome sequencing techniques to detect genetic mutations.The results of the current research showed the emergence of several deletion and substitution mutations in the 28S gene in strains resistant to whitefly pesticides. Where the results showed DNA-Sequencing through the samples that were studied, which indicated that the herbicide has a mutagenic and not a lethal effect, as the pesticide with its three concentrations and its frequency showed negative effects and the occurrence of genetic mutations on the genome of the whitefly, that is, the greater the concentration of the pesticide and the frequency of its spraying on If the mutagenic effect of this pesticide decreases, that is, the percentage of concentration is inversely proportional to the distinct mutant bands, as the percentage of variation in the samples whose genetic sequence was read decreases as the concentration and frequency of the pesticide increases, and thus these treatments produced strains of whitefly that had high resistance to this pesticide.